Scrabble?! IMMUNOBLOTTING

Definitions of IMMUNOBLOTTING in various dictionaries:

The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. Synthetic or animal-derived antibodies are created that react with a specific target protein. The sample material undergoes protein denaturation, followed by gel electrophoresis. Next, the electrophoresis membrane is washed in a solution containing the specific antibody. The excess antibody is then washed off, and a secondary antibody that reacts with the first antibody is added. Through various methods such as staining, immunofluorescence, and radioactivity, the secondary antibody can then allow visualisation of the protein. Other related techniques include dot blot analysis, quantitative dot blot, immunohistochemistry, and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme