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immunoblotting
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| The use of immunoblots to analyse proteins |
| A technique for analysing or identifying proteins in a mixture, involving separation by electrophoresis followed by staining with antibodies. |
| Immunoblotting might refer to |
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The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract. * Synthetic or animal-derived antibodies are created that react with a specific target protein. The sample material undergoes protein denaturation, followed by gel electrophoresis. Next, the electrophoresis membrane is washed in a solution containing the specific antibody. The excess antibody is then washed off, and a secondary antibody that reacts with the first antibody is added. Through various methods such as staining, immunofluorescence, and radioactivity, the secondary antibody can then allow visualisation of the protein. * Other related techniques include dot blot analysis, quantitative dot blot, immunohistochemistry, and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme |